Journal: Journal of Advanced Research
Article Title: Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy
doi: 10.1016/j.jare.2024.10.030
Figure Lengend Snippet: Astragaloside IV treatment of LPS-induced ER-autophagy in cardiomyocytes through DUSP1-PHB2 interaction. (A-B) Laser confocal detection of the endoplasmic reticulum. Scale bar, 25 μm (C-E) Detection of Complex I/III/V activity; (F) Detection of VDAC1 transcription level; (G) Detection of mPTP open level; (H-M) Detection of transcription levels of genes related to the mitochondrial unfolded protein response; (N) Detection of expression levels of pan-apoptosis related regulatory proteins; (O) Detection of endoplasmic reticulum autophagy expression level; Scale bar, 125 μm (P-S) Detection of transcription levels of pan-apoptosis related genes.Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.
Article Snippet: NO: Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit E-EL-M3052, Elabscience, China);Mouse IL-10(Interleukin 10) ELISA Kit(E-EL-M0046, Elabscience, China);Mouse IL-17A(Interleukin 17A) ELISA Kit(E-EL-M0047, Elabscience, China);MDA(Malondialdehyde) ELISA Kit(E-EL-0060, Elabscience, China);A001-2-1 Superoxide Dismutase (SOD) Typing Test Kit(E-BC-K020-M, Elabscience, China);H545-1-1 Glutathione Peroxidase 4 (gpx4) Kit(E-BC-K096-S, Elabscience, China);Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit(E-BC-K834-M, Elabscience, China);Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K838-M, Elabscience, China);Cell Mitochondrial Complex III (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit(E-BC-K836-M, Elabscience, China);.
Techniques: Activity Assay, Expressing